Journal: Pharmaceutics

Article Title: Curcumin-Loaded Oil-Free Self-Assembled Micelles Inhibit the Influenza A Virus Activity and the Solidification of Curcumin-Loaded Micelles for Pharmaceutical Applications

doi: 10.3390/pharmaceutics14112422

Figure Lengend Snippet: ( A ) Cytotoxicity of curcumin and Cur-M. Cytotoxicity was estimated based on the cell survival rate. In brief, three cell lines (Madin–Darby canine kidney (MDCK), 293T, and A549) commonly used for influenza propagation were treated with various concentrations of curcumin, Cur-M, and DMSO for 24 h. Cell survival rate was monitored by total vital cells counts in each treated group relative to that of mock control sample. Results from three independent experiments were plotted. ( B ) The effect of the micelle on the plaque-forming ability of the influenza virus was initially determined by a time-of-drug-addition assay, in which the tested compounds were added to the culture medium at 16 h prior to infection (pre-treatment, I), at the time of virus infection (co-treatment, II), or after viral adsorption (post-entry treatment, III). ( C ) The effect of curcumin and Cur-M on viral production. MDCK cells were continuously treated with DMSO, 30 μM curcumin, or Cur-M during influenza A virus (IAV, 2000 pfu) infection. At 24 h post-infection (hpi), the yield of viral progenies was determined. The yields relative to the mock treatment from three independent experiments were plotted. Statistically significant differences compared with the DMSO group are indicated by * ( p < 0.05).

Article Snippet: Cells used in this study were purchased from the American Type Culture Collection (ATCC), including Madin-Darby canine kidney (MDCK) cells (ATCC, ATCCPTA-7909), human A549 cells (ATCC, CCL-185TM), and human embryonic kidney (HEK293T) cells (ATCC, CRL-3216TM).

Techniques: Control, Virus, Infection, Adsorption

Journal: Pharmaceutics

Article Title: Curcumin-Loaded Oil-Free Self-Assembled Micelles Inhibit the Influenza A Virus Activity and the Solidification of Curcumin-Loaded Micelles for Pharmaceutical Applications

doi: 10.3390/pharmaceutics14112422

Figure Lengend Snippet: Curcumin-loaded self-assembled micelles (Cur-M) exerted potent, but distinct, anti-influenza activity. MDCK cells were treated with Dulbecco’s modified eagle’s medium (DMEM, mock control), DMSO (solvent control), curcumin, or Cur-M at various times of influenza A virus (IAV) infection (100 pfu), including pre-treatment (I), co-treatment (II), or post-entry treatment (III). The effect on the plaque-forming ability (infectivity) is shown ( A ), and the results from three independent experiments are plotted ( B ). Statistically significant differences compared with the native curcumin group are indicated by * ( p < 0.05).

Article Snippet: Cells used in this study were purchased from the American Type Culture Collection (ATCC), including Madin-Darby canine kidney (MDCK) cells (ATCC, ATCCPTA-7909), human A549 cells (ATCC, CCL-185TM), and human embryonic kidney (HEK293T) cells (ATCC, CRL-3216TM).

Techniques: Activity Assay, Modification, Control, Solvent, Virus, Infection

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Weights of different extracts of F. racemosa bark and SWA results of those extracts with two different cell lines

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques: Control

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Images of the scratch wound assay of dichloromethane extract of F. racemosa on (a) BHK cells, A1- control at t = 0 h, A2- control at t = 24 h, A3- DCM extract at t = 0 h, A4- DCM extract at t = 24 h; (b) MDCK cells, B1- control at t = 0 h, B2- control at t = 24 h, B3- DCM extract at t = 0 h, B4- DCM extract at t = 24 h

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques: Scratch Wound Assay Assay, Control

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Percentage wound closure in the presence of column fractions of F. racemosa DCM extract on BHK and MDCK cell lines. (Bars represent the mean and confidence intervals of 95% of nine measurements in the three experiments)

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Weights of sub fractions of fraction HF 4 of hexanes extract and percentage wound closure of those fractions at 24 h with two different cell lines

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques: Control

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Percentage wound closure in the presence of column fractions of F. racemosa hexanes extract on BHK and MDCK cell lines. (Bars represent the mean and confidence intervals of 95% of nine measurements in the three experiments)

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Variation of percentage wound closure with concentration in the presence of lupeol ( 1 ), β-sitosterol ( 2 ) and asiaticoside (positive control) at 24 h: ( a ) BHK cell line, ( b ) MDCK cell line. (Bars represent the mean and confidence intervals of 95% of nine measurements in the three experiments)

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques: Concentration Assay, Positive Control

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Percentage wound closure in the presence of lupeol acetate ( 3 ) with time. a BHK cell line. b MDCK cell line. (Bars represent the mean confidence intervals of 95% of nine measurements in the three experiments)

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Dual function of active constituents from bark of Ficus racemosa L in wound healing

doi: 10.1186/s12906-018-2089-9

Figure Lengend Snippet: Thin Layer Chromatographic comparison of the EtOAc extracts showing hydrolysis of lupeol acetate during scratch wound assay (SWA) ( a ) BHK Cells. ( b ) MDCK cells. Spray reagent: Anisaldehyde spray reagent

Article Snippet: BHK 21(ATCC, CCL-10) and MDCK (ATCC, PTA 6502) cell cultures were established in the laboratory using standard in-vitro methods.

Techniques: Comparison, Scratch Wound Assay Assay

Journal: Cell Proliferation

Article Title: RNAi‐mediated inhibition of the desmosomal cadherin (desmoglein 3) impairs epithelial cell proliferation

doi: 10.1111/j.1365-2184.2011.00765.x

Figure Lengend Snippet: Modulation of Dsg3 expression affected cell proliferation in MDCK cells. (a) Western blotting of cell lysates extracted from cells with either overexpression (knock‐in: KI) or knockdown (KD) of Dsg3. (b) Confocal microscopy of MDCK cells with ectopic Dsg3 expression [myc‐tag in (i)] or with Dsg3 knockdown (iii). Images in (ii) were vector control cells. (c) Dispase fragmentation assay of MDCK cells with either vector or Dsg3 shRNAi transduction. Cells were grown to confluence before being treated with 2.4 units/ml dispase for about 30 min to detach epithelial sheets. These sheets were subjected to mechanical stress by pipetting five times with 1 ml tips. Epithelial fragments were quantified by ImageJ and increased fragments (up to 2‐fold) were seen in cells with Dsg3 silencing by shRNAi‐1 or shRNAi‐2, respectively. Data are averages of duplicates in each group. (d) Growth curve of matched MDCK cells with up‐ or down‐regulation of Dsg3. Cells with overexpression had higher proliferation, but those with Dsg3 knockdown exhibited lower growth rate compared to matched control cells.

Article Snippet: HuSH TM shRNA plasmids (29‐mer) used for transduction in MDCK cells were purchased from OriGene Technologies Inc (Rockville, MD, USA).

Techniques: Expressing, Western Blot, Over Expression, Knock-In, Confocal Microscopy, Plasmid Preparation, Transduction